📥 Sequence Input
Sequence Input Area
🕒 Recent Designs
🛡️ All history is stored locally in your browser.
⚙️ Optimization Settings
Design Objective
Current default first; alternatives and not-enabled objectives are disclosed separately.
Show alternative implemented objectives
Show experimental objectives not enabled
Host System
Codon Reference
Current production default only; comparator assets are retained for provenance and controlled analysis, not public selection.
Current default: NbeV1.1 HC CDS-derived i NbeV1.1 high-confidence CDS-derived codon usage is the configured in-silico software default for N. benthamiana. It is not experimental validation or comparative biological-performance evidence.
Production software baseline for N. benthamiana; public reference overrides remain disabled.
Other packaged codon-reference assets are retained for provenance, reproducibility, and controlled internal sensitivity analysis only. They are not shown as public product choices, and public API/UI reference overrides remain unsupported.
Advanced Settings
Sites will be removed from CDS by synonymous substitution where possible.
Optimizing sequence...
Sequence Checks
📊 Optimization Results
Ready to design.
Optimization will unlock:
📊 Visualization
Interactive GC Distribution Chart
📈 Comparison
Pre vs Post Optimization Metrics
🕒 Tracking
Local History & Session Sync
🧬 Sequence Checks
Live DNA Sequence Scrutiny
CDS DESIGN COMPLETE
N. benthamiana specific logic applied.
| Metric | Original | Optimized |
|---|---|---|
| Sequence Length | - | - |
| GC Content | - | - |
| CAI Score | - | - |
| Codon Changes | N/A | - |
| AA Preserved | — | ✅ 100% |
GC Distribution (50bp Window) — ■ Reference Band
CAI Score
-
GC % Content
-
PolyA Signals Found
-
Host Profile
-
View Predicted Structure
Opens external structure prediction services. Your sequence will be passed to the external service via URL.